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Figure 1: (a) Steps of method of preparation of autologous noncultured dermal cell suspension. Removal of epidermis by motorized dermabrader (b) Excision elliptical biopsy from covered site with complete removal of fat from the dermal tissue (c) Minced dermal tissue (d) Addition of collagenase to minced dermal tissue (e) Incubation in CO2 incubator for 4 h (f) Enzymatically digested dermal tissue (g) Centrifugation at 3000 rpm for 10 min (h) Final re-suspended dermal cell suspension

Figure 1: (a) Steps of method of preparation of autologous noncultured dermal cell suspension. Removal of epidermis by motorized dermabrader (b) Excision elliptical biopsy from covered site with complete removal of fat from the dermal tissue (c) Minced dermal tissue (d) Addition of collagenase to minced dermal tissue (e) Incubation in CO2 incubator for 4 h (f) Enzymatically digested dermal tissue (g) Centrifugation at 3000 rpm for 10 min (h) Final re-suspended dermal cell suspension