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Year : 2019  |  Volume : 85  |  Issue : 6  |  Page : 578-589

Evaluation of anti-gal enzyme-linked immunosorbent assay for the diagnosis of Indian post-kala-azar dermal leishmaniasis

Souvik Datta1, Manab Ghosh2, Somenath Sarkar3, Bibhuti Saha2, Sumi Mukhopadhyay1 
1 Department of Laboratory Medicine, School of Tropical Medicine, Kolkata, West Bengal, India
2 Department of Tropical Medicine, School of Tropical Medicine, Kolkata, West Bengal, India
3 Department of Dermatology, Bankura Sammilani Medical College, Government of West Bengal, Kenduadihi, Bankura, West Bengal, India

Correspondence Address:
Dr. Sumi Mukhopadhyay
Department of Laboratory Medicine, School of Tropical Medicine, 108 Chittaranjan Avenue, Kolkata - 700 073, West Bengal
India

Background: Elimination of kala azar from India is challenging as there are potential reservoirs of Leishmania donovani in patients with post-kala-azar dermal leishmaniasis (PKDL). The vast repertoire of carbohydrate moieties on L. donovani is known to elicit specific and strong humoral responses in patients with kala azar. Aim: The present study was undertaken to evaluate the diagnostic performances of anti-gal antibodies using enzyme-linked immunosorbent assay for successful serological diagnosis of PKDL in Indian patients and to differentiate cases of past cured visceral leishmaniasis infections. Methods: We developed Gal enzyme-linked immunosorbent assay to measure specific anti-gal IgG isotype in the sera of 71 Indian patients with PKDL. The diagnostic efficacy of the newly developed assay was evaluated for precision, sensitivity and accuracy. Results: Gal2 enzyme-linked immunosorbent assay revealed three-fold increased anti-gal titers in 71 patients with active PKDL compared to controls. Subclass enzyme-linked immunosorbent assay analysis further revealed enhanced IgG2 and IgG3 anti-gal titers in patients with PKDL compared to control subjects. The rank order for specificity and sensitivity for IgG subclasses was IgG3>IgG2>IgG4>IgG1. The area under the curve values of 0.98 and 0.99 were obtained for IgG and IgG3 Gal2 enzyme-linked immunosorbent assays respectively. Overall sensitivity and specificity were 95.7% (95% CI: 88.1–99.1) and 98.1% (95% confidence interval: 90.1–99.9), and 98.5% (95% CI: 92.4–99.9) and 98.1% (95% CI: 90.1–99.9), respectively. Intra-assay coefficient of variation was 1.5% and inter-assay coefficient of variation was 11.7%. Limitations: The Gal2 enzyme-linked immunosorbent assay needs to be further investigated in mass surveys. Conclusion: Taken together, anti-gal titers detected through Gal2 enzyme-linked immunosorbent assay can serve as an effective diagnostic tool in disease elimination setting and help in better case management in endemic districts.


How to cite this article:
Datta S, Ghosh M, Sarkar S, Saha B, Mukhopadhyay S. Evaluation of anti-gal enzyme-linked immunosorbent assay for the diagnosis of Indian post-kala-azar dermal leishmaniasis.Indian J Dermatol Venereol Leprol 2019;85:578-589


How to cite this URL:
Datta S, Ghosh M, Sarkar S, Saha B, Mukhopadhyay S. Evaluation of anti-gal enzyme-linked immunosorbent assay for the diagnosis of Indian post-kala-azar dermal leishmaniasis. Indian J Dermatol Venereol Leprol [serial online] 2019 [cited 2019 Nov 17 ];85:578-589
Available from: http://www.ijdvl.com/article.asp?issn=0378-6323;year=2019;volume=85;issue=6;spage=578;epage=589;aulast=Datta;type=0


 

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