Evaluation of anti-gal enzyme-linked immunosorbent assay for the diagnosis of Indian post-kala-azar dermal leishmaniasis
Souvik Datta1, Manab Ghosh2, Somenath Sarkar3, Bibhuti Saha2, Sumi Mukhopadhyay1
1 Department of Laboratory Medicine, School of Tropical Medicine, Kolkata, West Bengal, India
2 Department of Tropical Medicine, School of Tropical Medicine, Kolkata, West Bengal, India
3 Department of Dermatology, Bankura Sammilani Medical College, Government of West Bengal, Kenduadihi, Bankura, West Bengal, India
Background: Elimination of kala azar from India is challenging as there are potential reservoirs of Leishmania donovani in patients with post-kala-azar dermal leishmaniasis (PKDL). The vast repertoire of carbohydrate moieties on L. donovani is known to elicit specific and strong humoral responses in patients with kala azar.
Aim: The present study was undertaken to evaluate the diagnostic performances of anti-gal antibodies using enzyme-linked immunosorbent assay for successful serological diagnosis of PKDL in Indian patients and to differentiate cases of past cured visceral leishmaniasis infections.
Methods: We developed Gal enzyme-linked immunosorbent assay to measure specific anti-gal IgG isotype in the sera of 71 Indian patients with PKDL. The diagnostic efficacy of the newly developed assay was evaluated for precision, sensitivity and accuracy.
Results: Gal2 enzyme-linked immunosorbent assay revealed three-fold increased anti-gal titers in 71 patients with active PKDL compared to controls. Subclass enzyme-linked immunosorbent assay analysis further revealed enhanced IgG2 and IgG3 anti-gal titers in patients with PKDL compared to control subjects. The rank order for specificity and sensitivity for IgG subclasses was IgG3>IgG2>IgG4>IgG1. The area under the curve values of 0.98 and 0.99 were obtained for IgG and IgG3 Gal2 enzyme-linked immunosorbent assays respectively. Overall sensitivity and specificity were 95.7% (95% CI: 88.1–99.1) and 98.1% (95% confidence interval: 90.1–99.9), and 98.5% (95% CI: 92.4–99.9) and 98.1% (95% CI: 90.1–99.9), respectively. Intra-assay coefficient of variation was 1.5% and inter-assay coefficient of variation was 11.7%.
Limitations: The Gal2 enzyme-linked immunosorbent assay needs to be further investigated in mass surveys.
Conclusion: Taken together, anti-gal titers detected through Gal2 enzyme-linked immunosorbent assay can serve as an effective diagnostic tool in disease elimination setting and help in better case management in endemic districts.
Dr. Sumi Mukhopadhyay
Department of Laboratory Medicine, School of Tropical Medicine, 108 Chittaranjan Avenue, Kolkata - 700 073, West Bengal
|How to cite this article:|
Datta S, Ghosh M, Sarkar S, Saha B, Mukhopadhyay S. Evaluation of anti-gal enzyme-linked immunosorbent assay for the diagnosis of Indian post-kala-azar dermal leishmaniasis.Indian J Dermatol Venereol Leprol 2019;85:578-589
|How to cite this URL:|
Datta S, Ghosh M, Sarkar S, Saha B, Mukhopadhyay S. Evaluation of anti-gal enzyme-linked immunosorbent assay for the diagnosis of Indian post-kala-azar dermal leishmaniasis. Indian J Dermatol Venereol Leprol [serial online] 2019 [cited 2019 Nov 17 ];85:578-589
Available from: http://www.ijdvl.com/article.asp?issn=0378-6323;year=2019;volume=85;issue=6;spage=578;epage=589;aulast=Datta;type=0