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Year : 2010  |  Volume : 76  |  Issue : 2  |  Page : 150-157

Direct immunofluorescence of skin biopsy: Perspective of an immunopathologist

Ranjana Walker Minz1, Seema Chhabra1, S Singh2, BD Radotra3, Bhushan Kumar4 
1 Department of Immunopathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
2 Department of Paediatrics, Postgraduate Institute of Medical Education and Research, Chandigarh, India
3 Department of Histopathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
4 Department of Dermatology, Postgraduate Institute of Medical Education and Research, Chandigarh, India

Correspondence Address:
Seema Chhabra
House No. 110, Sector 15/A, Chandigarh, U.T
India

Background: By direct immunofluorescence (DIF), presence of immune complexes in the skin biopsy at various locations such as the dermo-epidermal junction, dermal blood vessels, etc. help to arrive at a diagnosis. Aims: (1) To study the role of DIF in confirmation or exclusion of diseases involving skin vis-à-vis histopathology and clinical diagnosis, (2) to describe the annual spectrum of dermatologic conditions that present to a tertiary referral center and require DIF examination of skin biopsy for confirmation of diagnosis. Methods: A total of 267 biopsies received over a period of 16 months in the Department of Immunopathology were analyzed along with clinical and histopathological details and the correlation between them was studied. Results: DIF was positive in 204 skin biopsies. Of these, 127 biopsies showed good clinico-immuno-histopathological correlation. In 10 cases, only DIF could clinch the diagnosis. In another nine cases, immune deposits were noted, which were unexpected in light of clinical and histopathological diagnosis. The most common skin involvement was seen in vasculitides. DIF was, however, non-contributory in lesions like erythema multiformè, post Kala-azar dermal leishmaniasis, sarcoidosis, lupus vulgaris, pyoderma gangrenosum and prurigo nodularis. Conclusion: The DIF of skin in conjunction with histopathology gives the best diagnostic yield. It is invaluable in confirming the diagnosis of small vessel vasculitides and bullous lesions of skin and can be used as an additional tool to pinpoint the diagnosis of systemic and localized autoimmune diseases involving the skin.


How to cite this article:
Minz RW, Chhabra S, Singh S, Radotra B D, Kumar B. Direct immunofluorescence of skin biopsy: Perspective of an immunopathologist.Indian J Dermatol Venereol Leprol 2010;76:150-157


How to cite this URL:
Minz RW, Chhabra S, Singh S, Radotra B D, Kumar B. Direct immunofluorescence of skin biopsy: Perspective of an immunopathologist. Indian J Dermatol Venereol Leprol [serial online] 2010 [cited 2020 Jun 1 ];76:150-157
Available from: http://www.ijdvl.com/article.asp?issn=0378-6323;year=2010;volume=76;issue=2;spage=150;epage=157;aulast=Minz;type=0


 

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