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 ORIGINAL ARTICLE
Year : 2019  |  Volume : 85  |  Issue : 3  |  Page : 258--265

Melanocyte spheroids are formed by repetitive long-term trypsinization


1 Department of Dermatology, The Third Affiliated Hospital of Soochow University, Changzhou, China
2 Department of Dermatology, The First Affiliated Hospital of Bengbu Medical College, Anhui, China

Correspondence Address:
Dr. Ru-Zhi Zhang
Department of Dermatology, The Third Affiliated Hospital of Soochow University, 185 Juqian Road, Changzhou - 213003
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijdvl.IJDVL_275_17

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Background: Autologous melanocyte transplantation plays an important role in the treatment of vitiligo. Objective: Previous studies have indicated that, compared with melanocytes growing in monolayers, melanocyte spheroids have a better survival in growth factor- and serum-deprived conditions. Methods: Melanocyte spheroids were obtained from human epidermis by repetitive long-term trypsinization and maintained an aggregated morphology for a short period in certain conditions. Results: Melanocyte spheroids were capable of growing into normal dendritic melanocytes in monolayer when they were harvested and reinoculated in 24-well plates. Immunohistochemical analysis of the melanocyte spheroids revealed that they were positive for HMB45, a melanosome-specific marker. No melanomas occurred when melanocyte spheroids were transplanted into mice. Conclusion: Our study provides a promising approach for melanocyte transplantation to treat vitiligo.






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Online since 15th March '04
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