| ORIGINAL ARTICLE
|Year : 2019 | Volume
| Issue : 3 | Page : 258--265
Melanocyte spheroids are formed by repetitive long-term trypsinization
Di Li1, Ru-Zhi Zhang1, Hai-Xia Shi1, Yu-Hua Yang1, Ting Tian1, Li Wang2
1 Department of Dermatology, The Third Affiliated Hospital of Soochow University, Changzhou, China
2 Department of Dermatology, The First Affiliated Hospital of Bengbu Medical College, Anhui, China
Background: Autologous melanocyte transplantation plays an important role in the treatment of vitiligo.
Objective: Previous studies have indicated that, compared with melanocytes growing in monolayers, melanocyte spheroids have a better survival in growth factor- and serum-deprived conditions.
Methods: Melanocyte spheroids were obtained from human epidermis by repetitive long-term trypsinization and maintained an aggregated morphology for a short period in certain conditions.
Results: Melanocyte spheroids were capable of growing into normal dendritic melanocytes in monolayer when they were harvested and reinoculated in 24-well plates. Immunohistochemical analysis of the melanocyte spheroids revealed that they were positive for HMB45, a melanosome-specific marker. No melanomas occurred when melanocyte spheroids were transplanted into mice.
Conclusion: Our study provides a promising approach for melanocyte transplantation to treat vitiligo.
Dr. Ru-Zhi Zhang
Department of Dermatology, The Third Affiliated Hospital of Soochow University, 185 Juqian Road, Changzhou - 213003
Source of Support: None, Conflict of Interest: None
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