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| ORIGINAL ARTICLE
|Year : 2018 | Volume
| Issue : 3 | Page : 269--274
Protective effects of glutamine on human melanocyte oxidative stress model
Liya Jiang1, Zhen Guo2, Yulong Kong3, Jianhua Liang4, Yi Wang5, Keyu Wang6
1 Department of Dermatology, Qilu Hospital, Shandong University, Jinan, 250012; Department of Dermatology, Liaocheng People's Hospital, Liaocheng, 252000, P. R. China
2 Department of General Surgery, Liaocheng People's Hospital, Liaocheng, 252000, P. R. China
3 Department of Dermatology, Qilu Hospital, Shandong University, Jinan, 250012; Department of Dermatology, Affiliated Nanjing Jiangbei Hospital, Medical College of Southeast University, Nanjing, 210048, P. R. China
4 Department of Dermatology, Liaocheng People's Hospital, Liaocheng, 252000, P. R. China
5 Department of Dermatology, Jinan Central Hospital Affiliated to Shandong University, Jinan, 250012, P. R. China
6 Department of Dermatology, Qilu Hospital, Shandong University, Jinan, 250012, P. R. China
Background: Vitiligo is a disorder caused by the loss of the melanocyte activity on melanin pigment generation. Studies show that oxidative-stress induced apoptosis in melanocytes is closely related to the pathogenesis of vitiligo. Glutamine is a well known antioxidant with anti-apoptotic effects, and is used in a variety of diseases. However, it is unclear whether glutamine has an antioxidant or anti-apoptotic effect on melanocytes.
Aims: The aim of this study was to investigate the protective effects of glutamine on a human melanocyte oxidative stress model.
Methods: The oxidative stress model was established on human melanocytes using hydrogen peroxide. The morphology and viability of melanocytes, levels of oxidants [reactive oxygen species and malondialdehyde], levels of antioxidants [superoxide dismutase and glutathione-S-transferase], and apoptosis-related indicators (caspase-3, bax and bcl-2) were examined after glutamine exposure at various concentrations. Expressions of nuclear factor-E2-related factor 2, heme oxygenase-1, and heat shock protein 70 were detected using western blot technique after glutamine exposure at various concentrations.
Results: Our results demonstrate that pre-treatment and post-treatment with glutamine promoted melanocyte viability, increased levels of superoxide dismutase, glutathione-S-transferase and bcl-2, decreased levels of reactive oxygen species, malondialdehyde, bax and caspase-3, and enhanced nuclear factor-E2-related factor 2, heme oxygenase-1, and heat shock protein 70 expression in a dose dependent manner. The effect of pre-treatment was more significant than post-treatment, at the same concentration.
Limitations: The mechanisms of glutamine activated nuclear factor-E2-related factor 2 antioxidant responsive element signaling pathway need further investigation.
Conclusions: Glutamine enhances the antioxidant and anti-apoptotic capabilities of melanocytes and protects them against oxidative stress.
Dr. Keyu Wang
Department of Dermatology, Qilu Hospital, Shandong University, Jinan 250012
P. R. China
Source of Support: None, Conflict of Interest: None
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