IADVL
Indexed with PubMed and Science Citation Index (E) 
 
Users online: 5191 
     Home | Feedback | Login 
About Current Issue Archive Ahead of print Search Instructions Online Submission Subscribe What's New Contact  
  Navigate here 
  Search
 
  
 Resource links
   Similar in PUBMED
   Article in PDF (926 KB)
   Citation Manager
   Access Statistics
   Reader Comments
   Email Alert *
   Add to My List *
* Registration required (free)  

 
  In this article
   References
   Article Figures

 Article Access Statistics
    Viewed4584    
    Printed69    
    Emailed3    
    PDF Downloaded95    
    Comments [Add]    
    Cited by others 1    

Recommend this journal

 


 
 Table of Contents    
NET LETTER
Year : 2011  |  Volume : 77  |  Issue : 2  |  Page : 252-253

Mycobacterium leprae in ocular tissues: Histopathological findings in experimental leprosy


1 Federal University of Espírito Santo, Vitória-ES, Brazil
2 Federal University of Espírito Santo, Vitória-ES, Brazil;London School of Hygiene and Tropical Medicine, London-UK
3 Lauro Souza Lima Institute, Bauru-SP, Brazil

Date of Web Publication8-Mar-2011

Correspondence Address:
Patrícia Deps
Departmento de Medicina Social, Centro de Ciências da Saúde, Universidade Federal do Espirito Santo. Av Marechal Campos, 1468. Vitória-ES, Brazil

Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0378-6323.77490

Rights and Permissions



How to cite this article:
Cardozo AV, Deps P, Antunes JM, Andréa de F B, Rosa PS. Mycobacterium leprae in ocular tissues: Histopathological findings in experimental leprosy. Indian J Dermatol Venereol Leprol 2011;77:252-3

How to cite this URL:
Cardozo AV, Deps P, Antunes JM, Andréa de F B, Rosa PS. Mycobacterium leprae in ocular tissues: Histopathological findings in experimental leprosy. Indian J Dermatol Venereol Leprol [serial online] 2011 [cited 2019 Aug 24];77:252-3. Available from: http://www.ijdvl.com/text.asp?2011/77/2/252/77490


Sir,

Leprosy is disease of systemic manifestations with a high level of ocular complications. [1] Inside the eyes, the anterior segment is the most common area where Mycobacterium leprae is found since it is the coldest part in the eye bulb. In experimental studies, it was shown that the temperature of the eye anterior segment is 3°C colder than the environment. [2] Additionally, M. leprae has been found in the iris, part of the eye bulb where it would be safe from the action of systemic drugs used for the treatment of leprosy. [2] The eyes are also frequently studied for complications related to leprosy, being the important criteria for disability grade assessment. [2] M. leprae has not been artificially cultivated yet. However, experimental leprosy has been successfully induced in animals such as mice and armadillos, which contributed mainly to improve the knowledge regarding leprosy immunology and pathology, biology of M. leprae and drug tests for leprosy. [3],[4] Very few experimental studies have been published disclosing the location of M. leprae in eye tissues; moreover, almost all were detected after the inoculation of the bacillus in the cornea stroma.

A nine banded armadillo was inoculated with M. leprae at a concentration of 10 8 bacilli/ml, through the intracardiac and subcutaneous (thighs) route. The bacilli were obtained from an untreated lepromatous leprosy patient. The study was carried out at Instituto Lauro de Souza Lima (ILSL), Bauru-SP, Brazil, that has legal requirements to keep the armadillos captive for use in scientific research . This study was granted approval by ILSL ethical committee for research. Nodules were observed in the inoculation site after 13 months and systemic leprosy dissemination was observed within 24 months, presenting enlarged inguinal lymph nodes and decrease in the hematocrit. Anesthesia with intramuscular tiletamine/zolazepam 5.0 mg/kg was performed followed by both enucleations. The now blind armadillo was euthanized at this time by exsanguination. The eye bulbs were stained with H&E for morphological analysis and Wade-Fite staining was done for identifying mycobacteria. In the orbital cavity bacilli were found, isolated and arranged in globi, in the ocular muscle fibers, near the sclera and conjunctiva. No local clinical inflammation features such as erythema and edema were observed [Figure 1] and [Figure 2]. These findings suggest that direct invasion of the eye ball is possible and may be the main route of involvement of eye in advanced cases of lepromatous leprosy. [1]
Figure 1: Histological section of half of the eye (H and E, ×5)

Click here to view
Figure 2:  Mycobacterium leprae Scientific Name Search rrow) in the fibromuscular tissue surrounding the sclera with no inflammatory reaction surrounding (Fite-Faraco, ×1000)

Click here to view


In advanced untreated lepromatous leprosy patients, it has been observed that the most frequent ocular and extra ocular alterations are madarosis, keratitis (because of the combination of the trichiasis, lagophthalmos and the loss of sensibility in the cornea, secondary to infections), conjuntivitis, scleritis, and espiscleritis, the cornea being the first ocular tissue to be involved in the human body. [2],[3] The lagophthalmos, a potentially sight threatening lesion, results from motor nerve lesions in leprosy, and is frequently related to type 1 lepra reaction. [5]

 
  References Top

1.Hobbs HE, Harman DJ, Rees JW, McDoughall AC. Ocular histopathology in animals experimentally infected with Mycobacterium leprae and M. lepraemurium. 1. M. leprae and M. lepraemurium infections in the mouse. 2. M. leprae infections in the 9-banded armadillo (Dasypus novemcinctus L.). Br J Ophthalmol 1978;62:516-24.   Back to cited text no. 1
    
2.Kanski JJ. Clinical Ophthalmology. Oxford: Butterworth-Heinemann; 1989.  Back to cited text no. 2
    
3.Job CK, Sanchez RM, Hastings RC. Manifestations of experimental leprosy in the armadillo. Am J Trop Med Hyg 1985;34:151-61.  Back to cited text no. 3
[PUBMED]  [FULLTEXT]  
4.Dethlefs R. Prevalence of ocular manifestations of leprosy in Port Moresby, Papua New Guinea. Br J Ophthalmol 1981;65:223-5.   Back to cited text no. 4
[PUBMED]  [FULLTEXT]  
5.Courttight P, Daniel E, Sundarrao, Ravanes J, Mengistu F, Belachew M, et al. Eye disease in multibacillary leprosy patients at the time of their leprosy diagnosis: Findings from the Longitudinal Study of Ocular Leprosy (LOSOL) in India, the Philippines and Ethiopia. Lepr Rev 2002;73:225-38.  Back to cited text no. 5
    


    Figures

  [Figure 1], [Figure 2]

This article has been cited by
1 Ocular leprosy
Andrzej Grzybowski,Malgorzata Nita,Marcos Virmond
Clinics in Dermatology. 2015; 33(1): 79
[Pubmed] | [DOI]



 

Top
Print this article  Email this article

    

Online since 15th March '04
Published by Wolters Kluwer - Medknow