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Year : 2010  |  Volume : 76  |  Issue : 2  |  Page : 196-197

Use of amniotic membrane in dermatology

1 Sri Ram Medical Centre, and G. Kuppuswamy Memorial Hospital, Coimbatore, India
2 Malabar Institute of Medical Sciences, Calicut, India

Date of Web Publication10-Mar-2010

Correspondence Address:
Annamma John
No: 30, Cornerstone house, Kanniya Nagar, TVS Nagar Road, Koundampalayam, Coimbatore 30
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0378-6323.60565

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How to cite this article:
John A, Oommen J. Use of amniotic membrane in dermatology. Indian J Dermatol Venereol Leprol 2010;76:196-7

How to cite this URL:
John A, Oommen J. Use of amniotic membrane in dermatology. Indian J Dermatol Venereol Leprol [serial online] 2010 [cited 2020 Jul 10];76:196-7. Available from:


Treatment of extensive epidermal damage such as toxic epidermal necrolysis, pemphigus or burns is a formidable challenge, usually managed by steroids with or without skin grafting. The authors have regularly been using amniotic membrane for burns since 1988, though not for dermatologic problems. Amniotic membrane has been used in burns and other wounds and ulcers since 1910. This is an ideal biological dressing in preventing fluid loss, protecting against desiccation, reducing contamination and relieving pain, providing ideal moist environment for healing. [1],[2],[3] Its bacteriostatic effects have been documented. [4],[5],[6] It has the advantage of ready availability at no extra cost to the patient, which is relevant in developing countries. [2],[7] Use of amniotic membrane in complex venous ulcers and intractable dermatological conditions have been reported. [8],[9]

We were forced to use amniotic membrane to cover extensive necrotic and painful ulcers in a patient who was on steroids for pemphigus, whose general condition precluded the possibility of anesthesia or surgery. While medical treatment started and continued on the basis of biopsy and culture reports, the pain and frequency of dressings forced us to try alternate methods, and we used amnion to cover the ulcers to reduce pain. We noted that patient was not only relieved of pain, but also had remarkable spontaneous healing. This experience encouraged us to try amnion for other dermatologic problems such as toxic epidermal necrolysis and Stevens Johnson syndrome.

A 48-year-old lady with pemphigus vulgaris, well controlled and in remission with 30 mg prednisolone developed painful tender nodules on the right leg, which broke down and coalesced into large ulcers with necrotic base. She was started on medical treatment based on the biopsy and culture reports. However, the ulcers continued to be extremely painful. Skin grafting was ruled out because of poor general condition, and for fear of non-healing donor site. The excruciating pain and the need for frequent change of dressings forced us to try amniotic membrane to cover the ulcers [Figure 1]. The patient reported immediate pain relief on application of amnion and there was significant reduction in the ulcer discharge, and so, frequency of dressings reduced considerably, and even the subsequent amnion dressing changes were significantly painless. After three months of treatment, healthy granulations were noted and most of the ulcers healed spontaneously within four months. The other small areas of ulcers were skin grafted to complete the healing [Figure 2].

A 58-year-old man was admitted with Stevens Johnson syndrome after carbamezepine ingestion. He developed large areas of denudation on the trunk, face and limbs. After washing these areas with sterile saline, amnion was applied on it and systemic steroids and antibiotics started. With amnion application he was free from pain, discomfort and discharge; the covered areas remained dry and ulcers healed in 17 days without any further dressing and he was discharged on the 17 th day.

Amniotic membranes obtained with sterile precaution from normal delivery cases tested negative for HIV and HbsAg, were cleaned and washed several times in sterile water to remove traces of blood, and then in sterile saline and heparin, and stored after adding 80 mg of gentamicin [1] at -20 degrees celsius. A few hours before application, this was thawed and applied with surgical sterility on the wound. A liberal frill of amnion was extended onto the normal surrounding skin and the limb kept elevated to let it dry.

Having seen encouraging results, we feel that amnion is useful for complex and extensive skin loss, particularly in developing countries where the cost of dressing material adds to the patient's burden. The following chart summarizes the uses of amnion, its advantages and disadvantages [Table 1] and [Table 2].

  References Top

1.Colocho G, Graham WP, Green AE, Matheson DW, Lynch D. Human amniotic membrane as a physiologic wound dressing. Arch Surg 1974;109:370-4.  Back to cited text no. 1      
2.Ravishanker R, Bath AS, Roy R. 'Amnion Bank' - use of long term glycerol preserved amniotic membrane for management of superficial and superficial partial thickness burns. Burns 2003;29:369-74.  Back to cited text no. 2  [PUBMED]    
3.Bennet JP, Mathews R, Faulk WP. Treatment of chronic ulceration of leg with human amnion. Lancet 1980;315:1153-6.  Back to cited text no. 3      
4.Burleson R, Eiseman B. Mechanism of antibacterial effect of biological dressings. Ann Surg 1973;177:181-7.  Back to cited text no. 4  [PUBMED]    
5.Quinby WC, Hoover HC, Scheflan M, Walters PT. Clinical trials of amniotic membrane in burn wound care. Plast Reconst Surg 1982;70:711-6.  Back to cited text no. 5      
6.Robson MC, Krizek TJ. The effect of Human amniotic membrane on the bacterial population of infected rat burns. Ann Surg 1973;177:144-9.  Back to cited text no. 6  [PUBMED]    
7.Torrati FG, Rossi GA, Ferreira E, et al. Analysis of cost of dressings in the care of burns patients. Burns 2000;26:289-93.  Back to cited text no. 7      
8.Mermet I, Pottier N, Sainthillier JM, Malugani C, Cairey-Remonnay S, Maddens S, et al. Use of amniotic membrane transplantation in the treatment of venous leg ulcers. Wound Repair Regen 2007;15:459-64.  Back to cited text no. 8  [PUBMED]    
9.Hasegawa T, Mizoguchi M, Haruna K, Mizuno Y, Muramatsu S, Suga Y, et al. Amnia for intractable skin ulcers with recessive dystrophic epidermolysis bullosa: Report of three cases. Dermatology 2007;34:328-32.  Back to cited text no. 9      


  [Figure 1], [Figure 2]

  [Table 1], [Table 2]

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