Indexed with PubMed and Science Citation Index (E) 
Users online: 5194 
     Home | Feedback | Login 
About Current Issue Archive Ahead of print Search Instructions Online Submission Subscribe What's New Contact  
    Next article
    Previous article
    Table of Contents

    Similar in PUBMED
    Search Pubmed for
    Search in Google Scholar for
  Related articles
    Citation Manager
    Access Statistics
    Reader Comments
    Email Alert *
    Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded12    
    Comments [Add]    

Recommend this journal


Year : 2001  |  Volume : 67  |  Issue : 5  |  Page : 246--250

Use of polymerase chain reaction (PCR) for detection of Chlamydia trachomatis infection in cervical swab samples

Institute for Research in Reproduction (ICMR), Jehangir Merwanji Street, Parel, Mumbai - 400 012, India

Correspondence Address:
Jayanti Mania-Pramanik
Institute for Research in Reproduction (ICMR), Jehangir Merwanji Street, Parel, Mumbai - 400 012
Login to access the Email id

Source of Support: None, Conflict of Interest: None

PMID: 17664762

Rights and PermissionsRights and Permissions

A polymerase chain reaction (PCR) based method has been set up for detection of Chlamydia trachomatis (C. trachomatis) infection in single cervical swab samples. A primer pair specific to the major outer membrane protein (MOMP) gene common to all serotypes of C. trachomatis was used. This method was validated for its sensitivity as well as specificity. A minimum Ing of DNA could be used for detection of the infection. Specificity of the method was confirmed by carrying out a sample dilution curve. The cervical swab samples analysed in the present study were in coded form for validation of the PCR with an established commercial ELISA (Chlamydiazyme). Both the sensitivity and specificity of PCR was 100% when the ELISA results of these samples were decoded. Thus, this PCR technique could be used for better diagnosis of C. trachomatis infection in comparison to the commercially available ELISA technique.

[FULL TEXT] [PDF Not available]*

Print this article     Email this article

Online since 15th March '04
Published by Wolters Kluwer - Medknow