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 ORIGINAL ARTICLE
Year : 2001  |  Volume : 67  |  Issue : 5  |  Page : 246--250

Use of polymerase chain reaction (PCR) for detection of Chlamydia trachomatis infection in cervical swab samples


Institute for Research in Reproduction (ICMR), Jehangir Merwanji Street, Parel, Mumbai - 400 012, India

Correspondence Address:
Jayanti Mania-Pramanik
Institute for Research in Reproduction (ICMR), Jehangir Merwanji Street, Parel, Mumbai - 400 012
India
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Source of Support: None, Conflict of Interest: None


PMID: 17664762

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A polymerase chain reaction (PCR) based method has been set up for detection of Chlamydia trachomatis (C. trachomatis) infection in single cervical swab samples. A primer pair specific to the major outer membrane protein (MOMP) gene common to all serotypes of C. trachomatis was used. This method was validated for its sensitivity as well as specificity. A minimum Ing of DNA could be used for detection of the infection. Specificity of the method was confirmed by carrying out a sample dilution curve. The cervical swab samples analysed in the present study were in coded form for validation of the PCR with an established commercial ELISA (Chlamydiazyme). Both the sensitivity and specificity of PCR was 100% when the ELISA results of these samples were decoded. Thus, this PCR technique could be used for better diagnosis of C. trachomatis infection in comparison to the commercially available ELISA technique.






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Online since 15th March '04
Published by Wolters Kluwer - Medknow