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ORIGINAL CONTRIBUTIONS
Year : 1999  |  Volume : 65  |  Issue : 2  |  Page : 69-71

A bacterial study of pyoderma in Belgaum




Correspondence Address:
M Jyothi Nagmoti


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Source of Support: None, Conflict of Interest: None


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  Abstract 

One hundred children with primary pyoderma and 50 healthy children were included in the study. The swabs collected from the lesions were cultured on various media. The colonies were identified by conventional methods. Commonest isolate was Staph. aureus (45%), followed by Strep. pyogens (35%), E. coli (5%), Citrobacter (1%) and Staph. and Strept. together (14%). Staphylococci showed highest resistance to ampicillin (85%), followed by penicillin (78%), tetracycline (40%) and ciprofloxacin (15%). Streptococci and other Gram-negative isolates were sensitive to most of the drugs. Most strains of staphylococci were nontypable (42.2%) suggesting the possible emergence of new strains. Among typable ones, phage group-1 was commonest.


Keywords: Pyoderma, Staph.Phage types, Drug resistance


How to cite this article:
Nagmoti M J, Patil C S, Metgud S C. A bacterial study of pyoderma in Belgaum. Indian J Dermatol Venereol Leprol 1999;65:69-71

How to cite this URL:
Nagmoti M J, Patil C S, Metgud S C. A bacterial study of pyoderma in Belgaum. Indian J Dermatol Venereol Leprol [serial online] 1999 [cited 2019 Sep 20];65:69-71. Available from: http://www.ijdvl.com/text.asp?1999/65/2/69/4763



  Introduction Top


The patients with cutaneous lesions present one of the most challenging and frequently rewarding problems in clinical practice; especially in school going children.[1] Most of such lesions are primary pyoderma. Primary pyoderma is a pyogenic infection of the skin (nondiseased) and its appendages. Most commonly these lesions are produced by staphylococcal and streptococcal species, and less commonly by other Gram-negative organisms. Though easily treatable the condition is known for complication like post streptococcoal glomerulonephritis.[2][3] Therefore timely recognition and a prompt bacterial diagnosis of such lesions is mandatory. Hence the present study was conducted with aim of isolation, identification and antibiotic susceptibility testing of the isolates.


  Materials and Methods Top


The study was conducted in the department of Microbiology, J.N. Medical College, Belagaum in collaboration with Civil Hospital Belgaum. Study group comprised of 100 children with primary pyoderma attending Dermatology OPD of Civil hospital, Belgaum.

The pus was collected on two sterile cotton swabs after puncturing a fresh closed lesion with a sterile needle. The specimens were transported to the laboratory and processed within 2 hours. One swab was used for smear examination after Grams, staining and another was used to put up culture on blood agar, Mac.Conkey's agar and crystal violet blood agar (1 in 50000 crystal violet in candle jar (5% CO2). The isolates were identified by standard conventional methods. Antibiogram of the isolates was performed using standard disc diffusion technique.[4]

All staphylococcal isolates were sent to Moulana Azad Medical College, New Delhi (Bacteriophage reference center) for bacteriophage typing. Serogrouping of streptococci were done at Christain Medical College, Vellore (Streptococcal reference center). Fifty skin swabs were taken from normal healthy children and they were processed similarly as in study group and isolates were identified.


  Results Top


The age and Sex relationship is shown in [Table - 1]. Most of the patients belonged to the age group of 1-4 years (45%). Boys were more commonly affected (62%) than girls (38%). Majority of patients belonged to lower socioeconomic status (69%) than middle (27%) and higher (4%). We could also elicit history of similar lesions in the family members in 21 % of cases. Common sites involved were face, scalp and upper limbs.

[Figure - 1] shows bacterial isolates from pus. Infection was due to single organism in 86% and was due to mixed (staph.and strepto) in 14% of cases. Commonest isolate was Staphylococcus aureus (45%) followed by Streptococcus pyogenes (35%) and others.

[Figure - 2] shows bacteriophage-types of staphylococcal isolates. Many belonged to untypable group (43%) suggesting the possible emergence of new strains. Among typable group -1 was commoest. All streptococcal isolates belonged to serogroup-A.

[Figure - 3] shows antibiogram of staphylococci. Maximum resistance was shown against ampicilin (85%) and penicillin (78%), Streptococci and Gram negative organisms were sensitive to most of the drugs.

There was significant skin carriage of Staph.aureus in control group (15%).


  Discussion Top


Primary pyoderma is a common health problem in children. The results of the present Study reveal that Staph.aureus and Strepto.pyogenes are major etiological agents of primary pyoderma in Belgaum.[1][3] Similar findings have been reported from the other workers from different parts of India.[5][7] Mixed infection due to Staph.aureus and Strepto.pyogenes is also common.[8][9] Large number of staphylococci were untypable, which could probably be due to emergence of new strains. Phage group 1 staphylococci are common cause of infection;[10] also they are common prevailing group of staphylococci according to phage reference center.

Prolonged staphylococcal carriage on skin could also be one of the causative factors for primary pyoderma.[11] High degree of penicillin resistance correlated well with wide spread use of penicillin in private practice and also to penicillinase producing staphylococci.[7][8][9]

Hence timely recognition, and prompt bacterial diagnosis of the cases is very important for the management and also to check the major complications.

 
  References Top

1.Price PB. The bacteriology of normal skin: a new quantitative test applied to a study of the bacterial flora and the disinfectant action of mechanical cleansing. J Infect Dis 1938;63:301-308.  Back to cited text no. 1    
2.Dillon HC, Reeves MS. Streptococcal immune response in nephritis after skin infection. Am J Med Assoc 1974;56:333-346.  Back to cited text no. 2    
3.Maerowitz M, Breton D. The bacteriological findings, streptococcal immune response, and renal complication in children with impetigo. Paediatrics 1965;35:393-399.  Back to cited text no. 3    
4.Colle FG, Miles RS, Watt B. Tests for identification of bacteria. J Gerald, Collee, Barrie P Marmion, editors. In: Mackie and MC. Cartney Practical Medical Microbiology. New York: Churchil Livingstone, 1996:131.  Back to cited text no. 4    
5.Pasricha A, Bhujwala RA, Sriniwas A. Bacterial study of pyoderma. Indian J Pad) Bact 1972;15:131-138.  Back to cited text no. 5    
6.Khare AK. A clinical and bacteriological study of pyoderma. Indian J Dermatol Venereol Leprol 1988;54:192-195.  Back to cited text no. 6    
7.Bhaskaran CS, Shyamasundara Rao, Krishnamurthy, P, et al. Bacteriological study of pyoderma. Indian J Dermatol Venereol Leprol 1979;45:162-168.  Back to cited text no. 7    
8.Ramani S V, Jayakar PA. Bacteriological studies of 100 cases of pyoderma. Indian J Dermatol Venereol Leprol 1980;46:5:282-287.  Back to cited text no. 8    
9.Rountree PM, Freeman BM. Med J Australia 1955;2:543-547.  Back to cited text no. 9    
10.Miles AA, Williams RED, Copper B C, et al. Nasal carrier state of staphylococci. J Path Bact 1944;56;513-518.  Back to cited text no. 10    
11.Bhujwala RA, Mohapatra LN. Bacteriophage pattern and antibioltic resistance of Staph. aureus. Indian J Path Bact 1972;15:66-72.  Back to cited text no. 11  [PUBMED]  [FULLTEXT]


    Figures

[Figure - 1], [Figure - 2], [Figure - 3]

    Tables

[Table - 1]



 

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