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   Abstract
   Introduction
   Material and Method
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SHORT COMMUNICATION
Year : 1993  |  Volume : 59  |  Issue : 1  |  Page : 5-7

Evaluation of relative potencies of topical corticosteroids with histamine pin-prick method




Correspondence Address:
BSN Reddy


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Source of Support: None, Conflict of Interest: None


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  Abstract 

Relative potencies of different topical steroids in two serial concentrations were evaluated over the flexor aspect of human forearm in 50 volunteers. The results indicated that the order of potency for different steroid solutions ranged from the lowest with hydrocortisone acetate to the highest with betamethasone dipropionate while other steroids occupied intermediate positions. Further, it was noted that the potency of steroids is enhanced significantly by increasing their concentration.


Keywords: Topical steroids, Histamine bioassay


How to cite this article:
Reddy B, Shantharaman R. Evaluation of relative potencies of topical corticosteroids with histamine pin-prick method. Indian J Dermatol Venereol Leprol 1993;59:5-7

How to cite this URL:
Reddy B, Shantharaman R. Evaluation of relative potencies of topical corticosteroids with histamine pin-prick method. Indian J Dermatol Venereol Leprol [serial online] 1993 [cited 2019 Sep 19];59:5-7. Available from: http://www.ijdvl.com/text.asp?1993/59/1/5/3865



  Introduction Top


Topical corticosteroids are indispensable in today's dermatologic practice. With the initial success of topical hydrocortisone observed in 1972 by Sulzberger and Witten [1] in many recalcitrant dermatoses, a large number of synthetic topical corticosteroids have been produced in recent years. These are being marketed in a variety of commercial formulations such as ointments, creams, lotions, gels, etc. Proper evaluation of their efficacy is extremely important to the clinician to provide optimum therapeutic response. The present work was undertaken to assess the relative potencies of different topical steroid solutions in alcohol using the histamine pin-prick bioassay.


  Material and Method Top


Topical steroid powders (listed below) were dissolved in either ethanol or methanol in two different serial concentrations and tested over the flexor aspect of human forearm in 50 healthy volunteers according to the histamine pin-prick method of Reddy and Singh. [2]

Hydrocortisone 1.00 and 2.0

Prednisolone 0.25 and 0.5% Dexamethasone sodium

phosphate 0.25 and 0.5%

Desoximetasone 0.05 and 0.1 Triamcinolone

acetonide 0.05 and 0.1 Betamethasone

17-valerate 0.05 and 0.1 Betamethasone

dipropionate 0.05 and 0.1 %


  Results Top


The data pertaining to the histamine wheal response of the skin over the control and steroid-applied sites are summarized in [Table - 1]. There is a statistically significant difference in the mean histamine response noted over the control and steroid sites on one hand and also between different steroid-treated sites themselves on the other hand when compared with hydrocortisone (P< 0.001). Further, it was noted that by increasing the concentration of the steroids, there is enhanced suppression of histamine response.


  Comments Top


Relative potencies of various topical steroids ranged from the lowest with hydrocortisone acetate to the highest with betamethasone dipropionate while other steroids occupied intermediate positions. These findings are in general agreement with similar earlier studies. [2],[3],[4]

The effect of concentration on the percutaneous penetration of the steroids noted in the vasoconstrictor bioassay reveals that there is an increased penetration of the steroid by increasing its concentration. [5] This is further established in the present study by the fact that the maximum suppression of histamine wheal response was seen with higher concentration of the steroids investigated. In fact, this has an important clinical bearing and clearly points out that, in general, increasing the concentration is one valuable method of enhancing the steroid efficacy in patients who are less corticosteroid responsive as suggested by Robertson and Maibach. [6] However, the information available in relation to the concentration and response for topical corticosteroids is meager and the concentration response curve flattens out rapidly after an initial rise, indicating a different optimal concentration for each steroid. [7],[8],[9] Further investigations are needed to find out the optimal concentration of different topical steroids to . produce maximum suppression of the histamine wheal response over the skin.

 
  References Top

1.Sulzberger MB, Witten VH. The effect of topically applied compund F in selected dermatoses. J Invest Dermatol 1952; 19: 101-2.  Back to cited text no. 1  [PUBMED]  
2.Reddy BSN, Singh G. A new model for human bioassay of topical corticosteroids. Br J Dermatol 1976; 94: 191-3  Back to cited text no. 2    
3.Singh PK, Singh G. Relative potency of topical corticosteroid preparations. Ind J Dermatol Venereol Leprol 1985; 51: 309-12.  Back to cited text no. 3    
4.Reddy BSN, Santharaman R. Comparative evaluation of topical corticosteroids. Ind J Dermatol Venereol Leprol 1992; 58: 23-5.  Back to cited text no. 4    
5.McKenzie AW, Stoughton RB. Method for comparing percutaneous absorption of steroids. Arch Dermatol 1962; 86: 608-10.  Back to cited text no. 5    
6.Robertson DB, Maibach HI. Topical corticosteroids. Int J Dermatol 1982; 21: 59-67.  Back to cited text no. 6  [PUBMED]  
7.Schlegel CA, Northam JI. Comparative anti inflammatory efficacy of topically applied steroids on human skin. Proc Soc Exp Biol Med 1959,100: 629- 32.  Back to cited text no. 7    
8.Shah VP, Peck CC, Shelly JP. Vascoconstriction - skin blanching assay for glucocorticosteroids - a critique. Arch Dermatol 1989; 125: 1558-61.  Back to cited text no. 8    
9.Stoughton RB, Wullich K. The same glucocorticoid in brand-name products. Arch Dermatol 1989; 125:1509-11.  Back to cited text no. 9  [PUBMED]  


    Tables

[Table - 1]



 

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