| |
|
| Year : 1966 | Volume
: 32
| Issue : 2 | Page : 41--51 |
A New Convenient Egg-enriched Medium For the Cultural Diagnosis of Gonorrhoea
CW Chacko, GM Nair
,
Correspondence Address:
C W Chacko
The gonococcus has been found to grow well in artificial culture in a basal medium of Trypsin-digest-Beef-Muscle extract Agar enriched with the hen's egg. This egg enrichment medium has been compared with the same basal medium enriched with Ascitic Fluid and the Bacto G C Medium Base supplemented with Bacto Haemoglobin and Yeast Concentrate, by assaying them in parallel series for the primary diagnostic culture isolation of the gonococcus, from male and femal patients. In preliminary trials, the egg enriched medium has been found to support the growth and maintenance of several local fresh strains of the gonococcus, as well as the other two conventional enrichment media, in current use. The Egg, apparently contains yet unnamed special nutrient factor or factors required by the gonococcus to grow in artificial culture. Egg can be obtained more conveniently anywhere than the other conventional source or growth factors from Blood, Serum, Ascitic fluid and Yeast Extract in quality and quantity. The technique for preparation of the egg medium described lends itself with ease, for application in practice, in bacteriological laboratories anywhere. Therefore, the egg medium suggests itself as a good alternative culture medium that maybe used with advantage in the routine diagnosis of gonorrhoea everywhere. The various aspects of the egg medium is discussed and it is offered for more extensive study of potential worth discovered in this study.
[PDF Not available]*
|
